Hi,

I am looking for a research position in the fields of molecular microbiology, genomics and host-pathogen interactions preferably in California (but will consider other locations). Please find below a summary of my resume. My email is: rposhns@yahoo.com

Thanks,

SP


I am:
• Skilled in a wide range of molecular biology techniques
• Proficient in gene expression techniques and assay development including microarray profiling
• Experienced in working with pathogenic bacteria and infectious agents
• Experienced in high-throughput data analysis of microarray results
• A goal oriented, enthusiastic, focused, and efficient researcher with a strong commitment toward completion of projects efficiently and effectively
• Experienced in academic, national lab and industrial biotech settings
• Experienced in multidisciplinary projects

Recent experience:

2005 Senior research associate Department of Chemical and Materials Engineering, San Jose State University, San Jose, CA
• Developing a Surface Plasmon Resonance (SPR) sensor for label-free detection of DNA and RNA hybridization to surface-immobilized single-stranded DNA
• Working on cloning and expression of GFP in the thermophile Bacillus stearothermophilus. Designed a methanol dehydrogenase'-'GFP gene fusion for the induction of GFP by methanol at high temperatures

2001-2004 Postdoctoral Researcher Lawrence Livermore National Laboratory, Livermore, CA
• Studied the genetic and physiological elements involved in the virulence of yersinia Pestis, the causative agent of the Plague using microarray profiling analysis
• Characterized the global transcriptional response of yersinia Pestis and Yersinia pseudotuberculosis to acid pH
• Developed RNA extraction and microarray assay protocols
• Discovered putative novel virulence-related genes
• Constructed vectors for over-expression of small peptides for detection and identification of pathogenic bacteria by Bioaerosol Mass Spectrometry (BAMS).

2000-2001 Visiting Research Scientist DuPont Company, Wilmington, DE.
• Studied the global transcriptional response of the entire E. coli genome following chemical insults, using microarray profiling analysis
• Constructed chromosomal knockout strains using a PCR based protocol
• Analyzed mRNA transcripts stability (RNA decay) using Real-Time quantitative PCR.